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- Title
Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood.
- Authors
Zamay, Galina S.; Kolovskaya, Olga S.; Ivanchenko, Tatiana I.; Zamay, Tatiana N.; Veprintsev, Dmitry V.; Grigorieva, Valentina L.; Garanzha, Irina I.; Krat, Alexey V.; Glazyrin, Yury E.; Gargaun, Ana; Lapin, Ivan N.; Svetlichnyi, Valery A.; Berezovski, Maxim V.; Kichkailo, Anna S.
- Abstract
We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.
- Subjects
LUNG cancer diagnosis; CANCER patients; CELL adhesion molecules; CELL lines; DNA; EPITHELIAL cells; IMMUNOGLOBULINS; METASTASIS; MICROSCOPY; STATISTICS; DATA analysis
- Publication
Cancers, 2019, Vol 11, Issue 3, p351
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers11030351