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- Title
Molecular analysis of the β-globin gene cluster haplotypes in a Sudanese population with sickle cell anaemia.
- Authors
ELDERDERY, A. Y.; MILLS, J.; MOHAMED, B. A.; COOPER, A. J.; MOHAMMED, A. O.; ELTIEB, N.; OLD, J.
- Abstract
Introduction: Sudan has a multiethnic population with a high frequency of Hb S, but little is known about the βS haplotypes in this population. Methods: Blood samples from Sudanese Hb SS individuals were taken at two locations. Family history, age, ethnicity and clinical symptoms were recorded for each subject. Hb S was investigated using cellulose acetate electrophoresis (CAE) and cation exchange-high performance liquid chromatography. Dried blood samples from 93 individuals were used for βS haplotype identification based on restriction fragment length polymorphism analysis for seven restriction sites. Results: Haplotypes could be assigned unequivocally to 143 chromosomes. Four of the five typical βS-globin haplotypes were identified. The most frequent was the Cameroon (35.0%), followed by the Benin (29.4%), the Senegal (18.2%) and the Bantu (2.8%). The Indian-Arab haplotype was not observed. Three atypical haplotypes were identified in 17 patients, occurring at a combined frequency of 14.6%. One of these, found at the high frequency of 11.8%, possibly represented a new Sudan haplotype. Conclusion: βS Haplotyes were demonstrated successfully from dried blood samples. A new haplotype is apparent in Sudan, in addition to the four African haplotypes.
- Subjects
SUDAN; SICKLE cell anemia; ELECTROPHORESIS; ETHNIC groups; GENE amplification; GENES; GENETIC polymorphisms; INTERVIEWING; LIQUID chromatography; LONGITUDINAL method; POLYMERASE chain reaction; QUESTIONNAIRES; RESEARCH funding; DESCRIPTIVE statistics; GENETICS
- Publication
International Journal of Laboratory Hematology, 2012, Vol 34, Issue 3, p262
- ISSN
1751-5521
- Publication type
Article
- DOI
10.1111/j.1751-553X.2011.01388.x