We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The Protective Effect of Estrogen Receptor (ER)-beta Agonist on Cardiac Function Following Trauma-Hemorrhage: Downregulation of Cardiomyocyte L-Type Calcium Current (ICa-L).
- Authors
Shaolong Yang; Luyun Zou; Shunhua Hu; Chatham, John C.; Bland, Kirby I.; Machase, Richard B.; Chaudry, Irshad H.
- Abstract
Although 17β-estradiol (E2) administration after trauma-hemorrhage (T-H) produces salutary effects on cardiac function, the precise mechanism remains unknown. Additional studies have shown that cardiomyocyte Ica-L increased after T-H and the protective effect of E2 on cardiac function correlated with the attenuation of cardiomyocyte Ica-L. Although E2 produces various salutary effects, it is not known whether ER-α agonist (PPT) or β agonist (DPN) also attenuates cardiomyocyte Ica-L. Male rats (250-275 g) underwent T-H (removal of 60% of the circulating blood and fluid resuscitation after 90 min of hypotension) and received PPT, DPN (10 µg/kg, IV) or Cyclodextrin (20.7 mg/kg, IV, control) during resuscitation. Left ventricular (LV) performance was measured at 24 h and cardiomyocytes were then isolated. The whole-cell patch-clamp technique was employed to measure cardiomyocyte Ica-L. The results indicated that LV performance was decreased and cardiomyocyte Ica-L increased significantly after T-H; however, DPN restored cardiac function and attenuated the increase of cardiomyocyte Ica-L. In contrast, the beneficial effects of PPT on cardiac function did not reach a significance level. Thus, the salutary effect of E2 following trauma-hemorrhage appears to be primarily mediated via the ER-β receptor and may be a consequence of attenuation of cardiomyocyte Ica-L
- Subjects
ESTRADIOL; HEART cells; CARDIAC resuscitation; HEMORRHAGE; LEFT heart ventricle; LABORATORY rats
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1261
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fasebj.21.6.a1261