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- Title
Genomic identification of human vaccinia virus keratoconjunctivitis and its importance as a laboratory-acquired infection.
- Authors
Movahedi Motlagh, Zahra; Mokhtari, Azam; Mahzounieh, Mohammadreza; Motlagh, Zahra Movahedi
- Abstract
<bold>Context: </bold>Vaccinia virus (VACV) is a member of orthopoxvirus genus of the family Poxviridae. VACVs are enveloped, double-stranded DNA viruses. Several species of this family, for example, molluscum contagiosum, smallpox, deerpox, horsepox, rabbitpox, and VACVs may cause conjunctivitis.<bold>Aims: </bold>Given the high incidence of keratoconjunctivitis in Iran (approximately 3.6%-53.9%) and insufficient clinical diagnostic measures, laboratory tests for detection of its causes and determination of accurate keratoconjunctivitis/conjunctivitis prevalence due to different pathogens are essential.<bold>Settings and Design: </bold>In this research, conjunctival samples collected from 100 patients with keratoconjunctivitis signs were referred to an eye hospital of Iran.<bold>Subjects and Methods: </bold>After DNA extraction, polymerase chain reaction (PCR) was carried out for detection of VACV. PCR-positive products were further subjected to DNA sequencing.<bold>Statistical Analysis Used: </bold>The results were analyzed using Chi-square test.<bold>Results: </bold>In this study, 28% of the samples were positive and a statistically significant relationship obtained between working in medical or research laboratories and VACV prevalence (P < 0.05).<bold>Conclusions: </bold>This study showed a high rate of VACV keratoconjunctivitis, and therefore, further studies for its prevention and control are necessary.
- Subjects
IRAN; VACCINIA; ORTHOPOXVIRUSES; KERATOCONJUNCTIVITIS; MOLLUSCUM contagiosum; SMALLPOX; DNA analysis; LONGITUDINAL method; POLYMERASE chain reaction; POXVIRUS diseases; VIRUSES; GENOMICS; DISEASE incidence; DISEASE prevalence; CROSS-sectional method; RETROSPECTIVE studies; SEQUENCE analysis; VIRAL eye infections; DIAGNOSIS
- Publication
Indian Journal of Ophthalmology, 2016, Vol 64, Issue 11, p806
- ISSN
0301-4738
- Publication type
journal article
- DOI
10.4103/0301-4738.195592