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- Title
In vivo interaction between CDKA and eIF4A: a possible mechanism linking translation and cell proliferation
- Authors
Hutchins, Andrew P.; Roberts, Gethin R.; Lloyd, Clive W.; Doonan, John H.
- Abstract
In a proteomics-based screen for proteins interacting with cyclin-dependent protein kinase (CDK), we have identified a novel CDK complex containing the eukaryotic translation initiation factor, eIF4A. Reciprocal immunoprecipitations using antibodies against eIF4A indicate that the interaction is specific. The CDKA–eIF4A complex is abundant in actively proliferating and growing cells but is absent from cells that have ceased dividing. The CDKA–eIF4A complex contains kinase activity that is sensitive to the CDK-specific inhibitor roscovitine. This interaction points to a possible molecular mechanism linking cell proliferation with translational control.
- Subjects
PROTEOMICS; CARRIER proteins; EUKARYOTIC cells; IMMUNOGLOBULINS
- Publication
FEBS Letters, 2004, Vol 556, Issue 1-3, p91
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)01382-6