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- Title
Mathematical Description of the Enzymatic Activity of Proteins with Ionizable Groups Exhibiting Deviations from the Henderson-Hasselbalch Equation.
- Authors
Franco, Luis Fernando Mercier; Pessoa Filho, Pedro de Alcantara
- Abstract
The ionization equilibrium implied in the calculation of the specific activity is classically described through the Henderson-Hasselbalch equation. An extension for the description of anomalous ionization profiles using the Hill equation is presented in this communication. The proposed framework was applied to the description of the specific enzymatic activity curve as a function of pH of five enzymes presenting different ionization states in their active site. The developed equation improves the description of relative enzymatic curves that deviate from the bell curve predicted by the application of the Henderson-Hasselbalch equation, regardless of the ionization scheme related to the active site.
- Subjects
EQUATIONS; PROTEINS; ENZYMES; EQUILIBRIUM
- Publication
Applied Biochemistry & Biotechnology, 2022, Vol 194, Issue 3, p1221
- ISSN
0273-2289
- Publication type
Article
- DOI
10.1007/s12010-021-03700-y