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- Title
Comprehensive two-dimensional liquid chromatography with evaporative light-scattering detection for the analysis of triacylglycerols in Borago officinalis.
- Authors
Mondello, Luigi; Beccaria, Marco; Donato, Paola; Cacciola, Francesco; Dugo, Giovanni; Dugo, Paola
- Abstract
An optimized 2-D liquid chromatography (LC×LC) set-up, based on the different selectivities of a silver ion (Ag) and a non-aqueous reversed phase (NARP), employed in the first (D1) and the second dimension (D2), respectively, in combination with evaporative light-scattering detection (ELSD), has been developed for the analysis of the triacylglycerol (TAG) fraction in a Borago officinalis oil. The 2-D set-up, thanks to the complementary separation selectivity provided by the two columns, allowed to distribute 78 TAGs throughout the 2-D LC retention plane otherwise unachievable by 1-D LC.
- Publication
Journal of Separation Science, 2011, Vol 34, Issue 6, p688
- ISSN
1615-9306
- Publication type
Article
- DOI
10.1002/jssc.201000843