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- Title
Determinants of house dust, endotoxin, and β-(1→3)- D-glucan in homes of Danish children.
- Authors
Holst, G.; Høst, A.; Doekes, G.; Meyer, H. W.; Madsen, A. M.; Sigsgaard, T.
- Abstract
Little is known about the geographic variation and determinants of bacterial endotoxin and β -(1,3)- d-glucan in Danish house dust. In a population of 317 children, we: (i) described loads and concentrations of floor dust, endotoxin, and β-(1→3)- d-glucan and (ii) their correlations and (iii) assessed their determinants; (iv) Finally, we compared our findings with previous European studies. Bedroom floor dust was analyzed for endotoxin content by the kinetic limulus amoebocyte lysate assay and for β-(1→3)- d-glucan by the inhibition enzyme immunoassay. The parents answered questions regarding potential determinants. We found: geometric means (geometric standard deviations) 186 mg/m2 (4.3) for dust; 5.46 × 103 EU/m2 (8.0) and 31.1 × 103 EU/g (2.6) for endotoxin; and 142 μg/m2 (14.3) and 0.71 × 103 μg/g (7.3) for β-(1→3)- d-glucan. High correlations ( r > 0.75) were found between floor dust and endotoxin and β-(1→3)- d-glucan loads, while endotoxin and β-(1→3)- d-glucan concentrations were moderately correlated ( r = 0.36-0.41) with the dust load. Having a carpet was positively associated with dust load and with endotoxin and β-(1→3)- d-glucan concentrations. Pet keeping, dwelling type, and dwelling location were determinants of endotoxin concentrations. No other determinants were associated with β-(1→3)- d-glucan concentrations. Compared with other European studies, we found lower β-(1→3)- d-glucan loads and concentrations but higher endotoxin loads and concentrations suggesting a geographically determined different composition of Danish floor dust compared with other European regions.
- Subjects
DUST; ENDOTOXINS; BETA-glucans; INDOOR air pollution research; ENZYME-linked immunosorbent assay
- Publication
Indoor Air, 2015, Vol 25, Issue 3, p245
- ISSN
0905-6947
- Publication type
Article
- DOI
10.1111/ina.12143