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- Title
Applicability of a rapid stool antigen test, using monoclonal antibody to catalase, for the management of Helicobacter pylori infection.
- Authors
Shimoyama, Tadashi; Sawaya, Manabu; Ishiguro, Atsushi; Hanabata, Norihiro; Yoshimura, Tetsuro; Fukuda, Shinsaku
- Abstract
Background: Most monoclonal antibody-based stool antigen tests are of the 'send-out' format. Rapid Testmate pylori antigen (Rapid TPAg) uses monoclonal antibody and is an 'in-the-office' test. The aim of this study was to examine the usefulness of Rapid TPAg for the management of H. pylori infection. Methods: One-hundred and two consecutive patients who received H. pylori eradication therapy underwent both urea breath test (UBT) and Rapid TPAg at 5-6 weeks after finishing the eradication therapy. Stool samples were maintained at −20, 5, 25, and 40°C and subjected to Rapid TPAg after 1-7 days. Stool samples were also tested by enzyme immunoassay (EIA) to quantify antigenicity. Results: The agreement between Rapid TPAg and UBT in the evaluation of the results of H. pylori eradication treatment was 94.1%. The overall accuracy of Rapid TPAg and UBT to determine H. pylori eradication was 98.0 and 96.0%, respectively. The results of Rapid TPAg were not altered after storage of samples at −20 to 40°C for 7 days. Antigenicity quantified by EIA did not decrease significantly after 7 days. Conclusions: Rapid TPAg is a useful diagnostic test for immediate and accurate determination of the results of H. pylori eradication therapy. The antigenicity of stool sample suspensions was preserved for 7 days in the collection devices.
- Subjects
HELICOBACTER disease treatment; MONOCLONAL antibodies; HELICOBACTER pylori; GRAM-negative bacterial diseases; CATALASE test (Microbiology); BREATH tests; ENZYME-linked immunosorbent assay; DIAGNOSIS
- Publication
Journal of Gastroenterology, 2011, Vol 46, Issue 4, p487
- ISSN
0944-1174
- Publication type
Article
- DOI
10.1007/s00535-011-0371-4