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- Title
Biotransformation of soybean isoflavones by a marine Streptomyces sp. 060524 and cytotoxicity of the products.
- Authors
K. Hong; Y.-C. Song; J.-Y. Liu; R.-X. Tan
- Abstract
Abstract  A marine Streptomyces sp. 060524 capable of hydrolyzing the glycosidic bond of isoflavone glycosides, was isolated by detecting its β-glucosidase activity. 5 isoflavone aglycones were isolated from culture filtrates in soybean meal glucose medium. They were identified as genistein (1), glycitein (2), daidzein (3), 3â²,4â²,5,7-tetrahydroxyisoflavone (4), and 3â²,4â²,7-trihydroxyisoflavone (5), based on UV, NMR and mass spectral analysis. The Streptomyces can selectively hydroxylate at the 3â²-position in the daidzein and genistein to generate 3â²-hydroxydaidzein and 3â²-hydroxygenistein, respectively. The Strain biotransformed more than 90% of soybean isoflavone glycosides into their aglycones within 108 h. 3â²-hydroxydaidzein and 3â²-hydroxygenistein exhibited stronger cytotoxicity against K562 human chronic leukemia than daidzein and genistein.
- Subjects
ISOFLAVONES; BIOTRANSFORMATION (Metabolism); SOYBEAN; STREPTOMYCES; FUNCTIONAL groups; GLYCOSIDES; CELL-mediated cytotoxicity; MARINE microbiology; CHRONIC leukemia
- Publication
World Journal of Microbiology & Biotechnology, 2009, Vol 25, Issue 1, p115
- ISSN
0959-3993
- Publication type
Article