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- Title
MicroRNA 628 suppresses migration and invasion of breast cancer stem cells through targeting SOS1.
- Authors
Lin, Chenghui; Gao, Bin; Yan, Xuemao; Lei, Zixiong; Chen, Kebing; Li, Yuquan; Zeng, Qing; Chen, Zeqin; Li, Haomiao
- Abstract
Purpose: The purpose of this study is to evaluate the effects of miR-628 on migration and invasion of breast cancer stem cells (CSCs), which are essential for tumor recurrence and metastasis. Materials and methods: Quantitative reverse transcription-polymerase chain reaction was used to determine the expression of microRNAs and mRNAs. A subpopulation of CD44+/CD24- breast CSCs were sorted by flow cytometry. Transwell assays were used to evaluate cell migration and invasion. Luciferase reporter assays were performed to verify whether miR-628 targeted SOS Ras/Rac guanine nucleotide exchange factor 1 (SOS1). pcDNA3.1(+)-SOS1 was constructed for overexpressing SOS1 after transfection. Results: Compared with primary breast cancer cells, bone metastatic breast cancer cells showed significant downregulation of miR-628. The CD44+/CD24- breast CSC subpopulations in MDA-MB-231 and MCF-7 cell lines were analyzed and sorted. Transfection with an miR-628 mimic significantly suppressed the migration and invasion of these breast CSCs by targeting SOS1, which plays an essential role in epithelial-to-mesenchymal transition. Overexpression of SOS1 rescued miR-628-mediated migration and invasion by upregulating Snail and vimentin, and downregulating E-cadherin. Conclusion: miR-628 suppressed migration and invasion of breast CSCs of MDA-MB-231 and MCF-7 cells by directly targeting SOS1. Enhancement of miR-628 expression might be an effective strategy for managing breast cancer metastasis.
- Subjects
MICRORNA; BREAST cancer; STEM cells; REVERSE transcriptase polymerase chain reaction; FLOW cytometry
- Publication
OncoTargets & Therapy, 2018, Vol 11, p5419
- ISSN
1178-6930
- Publication type
Article
- DOI
10.2147/OTT.S164575