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- Title
Host Cell Lipid Bodies Triggered by Trypanosoma cruzi Infection and Enhanced by the Uptake of Apoptotic Cells Are Associated With Prostaglandin E<sub>2</sub> Generation and Increased Parasite Growth.
- Authors
D'Avila, Heloisa; Freire-de-Lima, Célio G.; Roque, Natalia R.; Teixeira, Livia; Barja-Fidalgo, Christina; Silva, Adriana R.; Melo, Rossana C. N.; DosReis, George A.; Castro-Faria-Neto, Hugo C.; Bozza, Patrícia T.
- Abstract
Lipid bodies (lipid droplets) are lipid-rich organelles with functions in cell metabolism and signaling. Here, we investigate the mechanisms of Trypanosoma cruzi-induced lipid body formation and their contributions to host-parasite interplay. We demonstrate that T. cruzi-induced lipid body formation in macrophages occurs in a Toll-like receptor 2-dependent mechanism and is potentiated by apoptotic cell uptake. Lipid body biogenesis and prostaglandin E2 (PGE2) production triggered by apoptotic cell uptake was largely dependent of αvβ3 and transforming growth factor-β signaling. T. cruzi-induced lipid bodies act as sites of increased PGE2 synthesis. Inhibition of lipid body biogenesis by the fatty acid synthase inhibitor C75 reversed the effects of apoptotic cells on lipid body formation, eicosanoid synthesis, and parasite replication. Our findings indicate that lipid bodies are highly regulated organelles during T. cruzi infection with roles in lipid mediator generation by macrophages and are potentially involved in T. cruzi-triggered escape mechanisms.
- Subjects
TRYPANOSOMA cruzi; APOPTOTIC protease-activating factor 1; PROSTAGLANDIN E1; BILAYER lipid membranes; GENETICS; PARASITES
- Publication
Journal of Infectious Diseases, 2011, Vol 204, Issue 6, p951
- ISSN
0022-1899
- Publication type
Article
- DOI
10.1093/infdis/jir432