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- Title
Sirolimus attenuates reduced-size liver ischemia-reperfusion injury but impairs liver regeneration in rats.
- Authors
Yuan-Xing Liu; Li-Ming Jin; Lin Zhou; Hai-Yang Xie; Guo-Ping Jiang; Hui Chen; Shu-Sen Zheng; Liu, Yuan-Xing; Jin, Li-Ming; Zhou, Lin; Xie, Hai-Yang; Jiang, Guo-Ping; Chen, Hui; Zheng, Shu-Sen
- Abstract
<bold>Background: </bold>Evidence has suggested that immunosuppressive drugs impact ischemia-reperfusion injury.<bold>Aims: </bold>The purpose of the present study was to evaluate the effect of sirolimus on hepatic injury and regeneration in a rat reduced-size liver ischemia-reperfusion model.<bold>Methods: </bold>Using a newly developed rat reduced-size liver ischemia-reperfusion injury model, the effects of sirolimus were evaluated by assessing liver cell apoptosis and aspartate aminotransferase, myeloperoxidase, and malondialdehyde levels. In addition, liver regeneration after sirolimus treatment was evaluated by measuring liver weight resumption and by the histological examination of bromodeoxyuridine and proliferating cell nuclear antigen expression.<bold>Results: </bold>Sirolimus significantly decreased liver cell apoptosis as well as tissue myeloperoxidase and malondialdehyde levels, but impaired postischemic liver regeneration. Ischemia-reperfusion-induced elevation of aspartate aminotransferase serum levels was significantly decreased by sirolimus.<bold>Conclusions: </bold>Despite an impairment of postischemic liver proliferation, sirolimus demonstrated beneficial amelioration of ischemia-reperfusion-induced liver injury in a reduced-size liver model in rats.
- Subjects
RAPAMYCIN; REDUCED-size transplantation; ISCHEMIA; TREATMENT of reperfusion injuries; LIVER regeneration; LABORATORY rats; WESTERN immunoblotting; ANALYSIS of variance; LIVER injuries; REPERFUSION injury; THERAPEUTICS; ANIMAL experimentation; ASPARTATE aminotransferase; CELL physiology; COMPARATIVE studies; EPITHELIAL cells; IMMUNITY; IMMUNOSUPPRESSIVE agents; RESEARCH methodology; MEDICAL cooperation; LIPID peroxidation (Biology); NEUTROPHILS; RATS; RESEARCH; TIME; EVALUATION research; PHARMACODYNAMICS; PHYSIOLOGY
- Publication
Digestive Diseases & Sciences, 2010, Vol 55, Issue 8, p2255
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-009-1002-2