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- Title
Changes in DNA-binding activity of transcription factors in the kidney of mice exposed to cadmium.
- Authors
Jin-Yong Lee; Chikage Mori; Maki Tokumoto; Masahiko Satoh
- Abstract
Cadmium (Cd) is a toxic heavy metal, long-term exposure to which causes renal damage associated with disruption in gene expression. Transcription factors whose activities were altered in the kidneys of mice exposed to Cd for 3 months were assessed using protein/DNA-binding assays. Female C57BL/6J mice were exposed to 300 ppm Cd in the diet for 3 months. Nuclear extracts of kidney were used for protein/DNA-binding assays. The concentration of Cd was approximately 100 ppm in mouse kidney, a level that did not induce renal toxicity. Among the 345 transcription factors evaluated, five transcription factors showed over a two-fold increase in their activities and 14 transcription factors showed a half-fold change in their activities after Cd exposure. These findings may provide new information about the causative transcription factors associated with Cd renal toxicity.
- Subjects
TRANSCRIPTION factors; METALLOTHIONEIN; CADMIUM; LABORATORY mice; KIDNEYS; NEPHROTOXICOLOGY; PHYTOCHELATINS
- Publication
Journal of Toxicological Sciences, 2021, Vol 46, Issue 3, p125
- ISSN
0388-1350
- Publication type
Article
- DOI
10.2131/jts.46.125