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- Title
Salidroside alleviates simulated microgravity-induced bone loss by activating the Nrf2/HO-1 pathway.
- Authors
Wang, Nan; Zuo, Zhuan; Meng, Tong; Liu, Yuliang; Zheng, Xiwei; Ma, Yongsheng
- Abstract
Background: Bone loss caused by microgravity exposure presents a serious threat to the health of astronauts, but existing treatment strategies have specific restrictions. This research aimed to investigate whether salidroside (SAL) can mitigate microgravity-induced bone loss and its underlying mechanism. Methods: In this research, we used hindlimb unloading (HLU) and the Rotary Cell Culture System (RCCS) to imitate microgravity in vivo and in vitro. Results: The results showed that salidroside primarily enhances bone density, microstructure, and biomechanical properties by stimulating bone formation and suppressing bone resorption, thereby preserving bone mass in HLU rats. In MC3T3-E1 cells cultured under simulated microgravity in rotary wall vessel bioreactors, the expression of osteogenic genes significantly increased after salidroside administration, indicating that salidroside can promote osteoblast differentiation under microgravity conditions. Furthermore, the Nrf2 inhibitor ML385 diminished the therapeutic impact of salidroside on microgravity-induced bone loss. Overall, this research provides the first evidence that salidroside can mitigate bone loss induced by microgravity exposure through stimulating the Nrf2/HO-1 pathway. Conclusion: These findings indicate that salidroside has great potential for treating space-related bone loss in astronauts and suggest that Nrf2/HO-1 is a viable target for counteracting microgravity-induced bone damage.
- Subjects
REDUCED gravity environments; BONE resorption; OXYGENASES; IN vitro studies; BONES; BIOMECHANICS; RESEARCH funding; BONE density; OSTEOBLASTS; BONE growth; EXTREMITIES (Anatomy); CELLULAR signal transduction; IN vivo studies; OXIDATIVE stress; SIMULATION methods in education; RATS; CELL lines; GENE expression; GLYCOSIDES; ANIMAL experimentation; ENVIRONMENTAL exposure; CELL differentiation; NUCLEAR factor E2 related factor
- Publication
Journal of Orthopaedic Surgery & Research, 2024, Vol 19, Issue 1, p1
- ISSN
1749-799X
- Publication type
Article
- DOI
10.1186/s13018-024-05030-1