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- Title
Multiplex PCR for the identification ofAgrobacteriumbiovar 3 strains.
- Authors
Kawaguchi, Akira; Sawada, Hiroyuki; Inoue, Kouji; Nasu, Hideo
- Abstract
A biovar 3-specific primer set Ab3-F3/Ab3-R4 was designed based on the comparison of sequences of the 16S rDNA region of agrobacteria and related rhizobia for rapid identification ofAgrobacteriumbiovar 3 strains. A 570-bp 16S rDNA fragment was amplified from cell lysates ofAgrobacteriumbiovar 3 strains by polymerase chain reaction (PCR) using Ab3-F3/Ab3-R4 primers. Discrimination ofAgrobacterium tumefaciensbiovar 3 fromAgrobacterium radiobacterbiovar 3 and ofAgrobacteriumbiovar 3 strains from otherAgrobacteriumstrains was done simultaneously using multiplex PCR with a mixture of two primer sets (Ab3-F3/Ab3-R4 and VCF3/VCR3) previously designed for thevirCregion of Ti-plasmid and Ri-plasmid.
- Subjects
AGROBACTERIUM tumefaciens; RECOMBINANT DNA; RHIZOBIACEAE; POLYMERASE chain reaction; AGROBACTERIUM; PLASMIDS
- Publication
Journal of General Plant Pathology, 2005, Vol 71, Issue 1, p54
- ISSN
1345-2630
- Publication type
Article
- DOI
10.1007/s10327-004-0160-5