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- Title
Screening of flavor compounds using Ucp1-luciferase reporter beige adipocytes identified 5-methylquinoxaline as a novel UCP1-inducing compound.
- Authors
Satoko Kawarasaki; Kazuki Matsuo; Hidetoshi Kuwata; Lanxi Zhou; Jungin Kwon; Zheng Ni; Haruya Takahashi; Wataru Nomura; Hisanori Kenmotsu; Kazuo Inoue; Teruo Kawada; Tsuyoshi Goto
- Abstract
Uncoupling protein 1 (UCP1) in brown or beige adipocytes is a mitochondrial protein that is expected to enhance whole-body energy expenditure. For the high-throughput screening of UCP1 transcriptional activity regulator, we established a murine inguinal white adipose tissue-derived Ucp1- luciferase reporter preadipocyte line. Using this reporter preadipocyte line, 654 flavor compounds were screened, and a novel Ucp1 expression-inducing compound, 5-methylquinoxaline, was identified. Adipocytes treated with 5-methylquinoxaline showed increased Ucp1 mRNA expression levels and enhanced oxygen consumption. 5-Methylquinoxaline induced Ucp1 expression through peroxisome proliferator-activated receptor γ coactivator 1 α (PGC1 α), and 5-methylquinoxaline-induced PGC1 α activation seemed to be partially regulated by its phosphorylation or deacetylation. Thus, our Ucp1 -luciferase reporter preadipocyte line is a useful tool for screening of Ucp1 inductive compounds.
- Subjects
LUCIFERASES; PEROXISOME proliferator-activated receptors; UNCOUPLING proteins; FAT cells; MITOCHONDRIAL proteins; FLAVOR
- Publication
Bioscience, Biotechnology & Biochemistry, 2022, Vol 86, Issue 3, p380
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbab216