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- Title
Tobacco rattle virus–induced gene silencing in Hevea brasiliensis.
- Authors
Li, Hui-Liang; Guo, Dong; Wang, Ying; Zhu, Jia-Hong; Qu, Long; Peng, Shi-Qing
- Abstract
Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS in rubber tree has not yet been reported. In this study, we described the efficient gene silencing in rubber tree by VIGS. The gene encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified in rubber tree genome. Small interfering RNAs from HbPDS and the silencing gene fragment were predicted and a length of 399 bp was selected to be tested. We showed that the tobacco rattle virus (TRV)-VIGS could induce effective HbPDS silencing in rubber tree. This study was the first to report VIGS in rubber tree. The present TRV-VIGS method could be used to perform reverse genetic approaches to identify unknown gene functions and might be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree.
- Subjects
HEVEA; SMALL interfering RNA; GENE silencing; RUBBER plants; PLANT gene silencing; TOBACCO; TREE planting
- Publication
Bioscience, Biotechnology & Biochemistry, 2021, Vol 85, Issue 3, p562
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbaa085