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- Title
Exogenous proline enhances the sensitivity of Tobacco BY-2 cells to arsenate.
- Authors
Nahar, Mst. Nur-E-Nazmun; Islam, Mohammad Muzahidul; Hoque, Md. Anamul; Yonezawa, Anna; Prodhan, Md. Yeasin; Nakamura, Toshiyuki; Nakamura, Yoshimasa; Munemasa, Shintaro; Murata, Yoshiyuki
- Abstract
Arsenic causes physiological and structural disorders in plants. Proline is accumulated as a compatible solute in plants under various stress conditions and mitigates stresses. Here, we investigated the effects of exogenous proline on tobacco Bright Yellow-2 (BY-2) cultured cells understress. Arsenate did not inhibit BY-2 cell growth at 40 and 50 μM but did it at 60 μM. Proline at 0.5 to 10 mM did not affect the cell growth but delayed it at 20 mM. At 40 μM, neither 0.5 mM nor 1 mM proline affected the cell growth but 10 mM proline inhibited it. In the presence of, 10 mM proline increased the number of Evans Blue-stained (dead) cells and decreased the number of total cells. Together, our results suggest that exogenous proline does not alleviate arsenate toxicity but enhances the sensitivity of BY-2 cells to arsenate. Enhancement of arsenate-induced cell growth inhibition in the presence of proline (Pro) in BY-2 cells. Arsenate-induced cell growth inhibition in the presence of 1 mM and 10 mM Pro but not in the presence of 0.5 mM Pro.
- Subjects
PHYSIOLOGICAL effects of tobacco; PROLINE
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 9, p1726
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1340088