We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A Semi-Synthetic Glycoconjugate Vaccine Candidate for Carbapenem-Resistant Klebsiella pneumoniae.
- Authors
Seeberger, Peter H.; Pereira, Claney L.; Khan, Naeem; Xiao, Guozhi; Diago‐Navarro, Elizabeth; Reppe, Katrin; Opitz, Bastian; Fries, Bettina C.; Witzenrath, Martin
- Abstract
Hospital-acquired infections are an increasingly serious health concern. Infections caused by carpabenem-resistant Klebsiella pneumoniae (CR- Kp) are especially problematic, with a 50 % average survival rate. CR- Kp are isolated from patients with ever greater frequency, 7 % within the EU but 62 % in Greece. At a time when antibiotics are becoming less effective, no vaccines to protect from this severe bacterial infection exist. Herein, we describe the convergent [3+3] synthesis of the hexasaccharide repeating unit from its capsular polysaccharide and related sequences. Immunization with the synthetic hexasaccharide 1 glycoconjugate resulted in high titers of cross-reactive antibodies against CR- Kp CPS in mice and rabbits. Whole-cell ELISA was used to establish the surface staining of CR- Kp strains. The antibodies raised were found to promote phagocytosis. Thus, this semi-synthetic glycoconjugate is a lead for the development of a vaccine against a rapidly progressing, deadly bacterium.
- Subjects
GLYCOCONJUGATES; NOSOCOMIAL infections; VACCINES; CARBAPENEMS; KLEBSIELLA pneumoniae; ANTIBIOTICS
- Publication
Angewandte Chemie, 2017, Vol 129, Issue 45, p14161
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201700964