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- Title
Laccase from a non-melanogenic, alkalotolerant γ-proteobacterium JB isolated from industrial wastewater drained soil.
- Authors
Bains, Jasleen; Capalash, Neena; Sharma, Prince
- Abstract
A Gram-negative, alkalotolerant bacterium, isolated from the soil continually drained with industrial wastewater and identified as gamma-proteobacterium by partial 16S rRNA sequence analysis, produced a polyphenol oxidase, which showed laccase but not tyrosinase activity. The organism grew well from pH 6 to 10 and produced laccase maximally at pH 10. The enzyme was stable from pH 3 to 10.6 for at least 24 h and was optimally active at 55 °C and pH 6.5 in a 5 min assay.
- Subjects
LACCASE; OXIDOREDUCTASES; ENZYMES; MICROBIAL enzymes; INDUSTRIAL wastes; BACTERIA; POLYPHENOL oxidase; POLYPHENOLS; OXIDASES
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 14, p1155
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1024569722413