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- Title
Desorption of cellulases from cotton powder.
- Authors
Azevedo, Helena; Pereira Ramos, Luiz; Cavaco-Paulo, Arthur
- Abstract
Cotton fabrics were treated with three different Trichoderma reesei cellulase preparations (total crude – TC, endoglucanase enriched – EG-rich, cellobiohydrolase enriched – CBH-rich) using mechanical agitation to produce cotton powder. Desorption of cellulase enzymes from the cotton powder was then performed by washing with buffer. After 3 washings most of the protein was desorbed from the cotton powder and the amount of sugars released in the latter washings was negligible. TC and CBH-rich preparations produced a finer cellulose powder than EGs. The desorption process caused a decrease in degree of polymerisation (DP) specially for the cotton treated with EGs and a marked increase in polydispersity (Pd) for all preparations.
- Subjects
TRICHODERMA; MONILIACEAE; COTTON textiles; CELLULASE; HYDROLASES; CATALYSTS
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 17, p1445
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1011619425613