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- Title
Expression of Bacillus sp. β-xylosidase gene (xylB) in Saccharomyces cerevisiae.
- Authors
Kim, Jong-Hyun; Kim, Byung-Woo; Yoon, Ki-Hong; Nam, Soo-Wan
- Abstract
β-Xylosidase gene (xylB) from Bacillus sp. was amplified and inserted between GAL10 promoter and GAL7 terminator. For the secretory production of xylB in Saccharomyces cerevisiae, in-frame fusion of the exoinulinase signal sequence (INU1s) of Kluyveromyces marxianus to the upstream of xylB was conducted. When a transformant of S. cerevisiae harboring the resulting plasmid was grown on galactose-containing medium, most of β-xylosidase activity was localized in the periplasmic space of yeast and a maximum total activity reached about 2.9 unit ml-1 at 42 h cultivation. The recombinant β-xylosidase was produced as an active dimer form.
- Subjects
BACILLUS sphaericus; SACCHAROMYCES cerevisiae; GENES; KLUYVEROMYCES marxianus; PLASMIDS; GALACTOSE
- Publication
Biotechnology Letters, 2000, Vol 22, Issue 12, p1025
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1005661909095