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- Title
Monitoring therapeutic effects in experimental stroke by serial USPIO-enhanced MRI.
- Authors
Marinescu M; Chauveau F; Durand A; Riou A; Cho TH; Dencausse A; Ballet S; Nighoghossian N; Berthezène Y; Wiart M; Marinescu, Marilena; Chauveau, Fabien; Durand, Anne; Riou, Adrien; Cho, Tae-Hee; Dencausse, Anne; Ballet, Sébastien; Nighoghossian, Norbert; Berthezène, Yves; Wiart, Marlène
- Abstract
<bold>Objectives: </bold>This study sought to evaluate whether the therapeutic effects of an anti-inflammatory drug such as minocycline could be monitored by serial ultrasmall superparamagnetic particles of iron oxide (USPIO)-enhanced MRI in experimental stroke.<bold>Methods: </bold>Mice received a three-dose minocycline treatment (n = 12) or vehicle (n = 12) after permanent middle cerebral artery occlusion. USPIOs were administered 5 h post-surgery. MRI was performed before, 24 h and 48 h post-USPIO administration. MRI endpoints were the extent of signal abnormalities on R2 maps (=1/T2) and quantitative R2 changes over time (∆R2). Post-mortem brains were prepared either for immunohistology (n = 16) or for iron dosage (n = 8).<bold>Results: </bold>As expected, treatment with minocycline significantly reduced infarct size, blood-brain barrier permeability and F4/80 immunostaining for microglia/macrophages. Areas of R2 maps > 35 ms(-1) also appeared significantly decreased in minocycline-treated mice (ANOVA for repeated measures, P = 0.017). There was a fair correlation between these areas and the amount of iron in the brain (R(2) = 0.69, P = 0.010), but no significant difference in ∆R2 was found between the two groups.<bold>Conclusions: </bold>This study showed that the extent of signal abnormalities on R2 maps can be used as a surrogate marker to detect minocycline effects in a murine experimental model of stroke.
- Publication
European Radiology, 2013, Vol 23, Issue 1, p37
- ISSN
0938-7994
- Publication type
journal article
- DOI
10.1007/s00330-012-2567-2