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- Title
Leukocyte Intracellular pH and Na/H Antiporter Activity in Uraemia and Type I Diabetes Mellitus.
- Authors
Poli de Figueiredo, C. E.; Ng, L. L.; Garrido, Maria C.; Davies, Joan E.; Ellory, J. C.; Hendry, B. M.
- Abstract
The leukocyte Na/H antiporter has been studied in patients with end-stage renal failure on maintenance haemodialysis. Thirteen non-diabetic haemodialysis patients (CRF group) and eight haemodialysis patients with diabetic nephropathy (CRF-DM group) were investigated. Measurements were made using the pH-sensitive fluorescent dye bis (carboxyethyl) carboxyfluorescein (BCECF). The initial intracellular pH (pHj), intracellular buffering capacity, and Na/H antiporter Vmax (at pHj = 6.0) have been recorded in bicarbonate-free solutions. The mean initial intracellular pH in the CRF group was 7.34 (SD 0.05, P<0.004) and this was significantly less than the CRFDM group (7.42, SD 0.07) and normal controls (7.43, SD 0.09, n = 25). The mean intracellular buffering capacity was normal in the CRF and CRF-DM groups. The mean Na/H antiporter Vmax was also normal in the CRF and CRF-DM groups (56.5, SD 9.9; and 56.8, SD 12.8, mmol/ 1 per min respectively compared to 55.2, SD 8.8, mmol/l per min in controls). These data are discussed with reference to the reported high values of Na/H antiporter Vmax in diabetic patients with early nephropathy. This abnormality does not appear to be present in end-stage diabetic nephropathy.
- Publication
Nephrology Dialysis Transplantation, 1991, Vol 6, Issue 9, p615
- ISSN
0931-0509
- Publication type
Article