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- Title
Oxidative/nitrosative stress and peroxiredoxin 2 are associated with grade and prognosis of human renal carcinoma.
- Authors
Soini, Y.; Kallio, J. P.; Hirvikoski, P.; Helin, H.; Kellokumpu-Lehtinen, P.; Kang, S. W.; Tammela, T. L. J.; Peltoniemi, M.; Martikainen, P. M.; Kinnula, V. L.
- Abstract
Peroxiredoxins (Prxs) 1–6 were assessed in 138 renal cell carcinomas (RCC) using immunohistochemistry and selected samples by Western blotting analysis. Oxidative/nitrosative damage was evaluated using nitrotyrosine immunoreactivity. The expressions of Prxs were correlated with tumor grade and survival and nitrotyrosine reactivity. Non-malignant kidney tubular cells showed positivity with variable intensity for all six Prxs. In RCCs, most cases were positive for Prxs 1 and 2, while only 15–20% of tumors showed expression for Prxs 3 and 4. Prx 2 was associated with tumors of a lower grade (p=0.009) and with a lower frequency of distant metastases (p=0.046). Patients with tumors expressing Prx2 had better prognosis (p=0.027). Instead, nitrotyrosine was significantly associated with high grade tumors (p=0.001). Compared with the non-malignant kidney tubular cells, low Prx expression in the tumor cells can make them more susceptible to oxidative damage. Prx 2 was more abundantly expressed in low grade tumors, suggesting that this protein could play a role in preventing the development of oxidative damage, which in turn can lead to the activation of pathways leading to aggressive tumors.
- Subjects
RENAL cell carcinoma; IMMUNOHISTOCHEMISTRY; TYROSINE; KIDNEY tubules; WESTERN immunoblotting; METASTASIS
- Publication
APMIS, 2006, Vol 114, Issue 5, p329
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1600-0463.2006.apm_315.x