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- Title
Bodipy-C12 在癌细胞过氧化物酶体中富集.
- Authors
高关刚; 盛园园; 张嘉杰; 刘佳明; 徐俐
- Abstract
Objective: To investigate and compare the subcellular localization of Bodipy-labeled lauric acid(Bodipy-C12) in cancer cells and normal cells. Methods: Various cancer cells and normal cells were cultured in medium with Bodipy-C12(1 μg/m L). We detected the signal of Bodipy-C12 in different organelles labeled by specific molecular maker proteins with using live-cell time-lapse confocal microscope in 5 minutes. Results: In a human hepatocellular carcinoma cell line HepG2, the signal of Bodpy-C12 was not only detected in lipid droplet and mitochondria, but it can also be enriched in peroxisomes. The co-localization of Bodpy-C12 signal with specific molecular protein markers, including Pex3-GFP, Pex14-GFP, GFP-Pex16, GFP-SKL and GFP-Pmp34, verified the existence of Bodpy-C12 in peroxisomes. Further, the enrichment of Bodpy-C12 in peroxisomes occured to more cancer cell lines such as colorectal cancer cell HCT116 and breast cancer cell MCF7. Differently, Bodpy-C12 signal was detected only in lipid droplet and mitochondria, but not in peroxisomes of normal cell lines such as 3 T3-L1, NRK, COS7. Conclusions: Bodipy-C12 signal existed in lipid droplet and mitochondria of normal cells and cancer cells, and it can be enriched in peroxisomes of cancer cells, but not in peroxisomes of normal cells, indicating a different lipid metabolism of the peroxisomes in cancer cells.
- Subjects
PEROXISOMES; CANCER cells; LAURIC acid; LIPID metabolism; CELL lines
- Publication
Progress in Modern Biomedicine, 2020, Vol 20, Issue 1, p1
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2020.01.001