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- Title
The Prokaryotic Community Structure of Oil-Contaminated Chernozem as Affected by the Introduction of Potassium Nitrate and Chloride.
- Authors
Vlasova, A. P.; Pavlov, K. V.; Morachevskaya, E. V.; Lipatov, D. N.; Pozdnyakov, L. A.; Manucharova, N. A.
- Abstract
The effect of potassium nitrate and chloride, which are a part of nitrogen fertilizers, on the structure of the metabolically active prokaryotic community of oil-contaminated chernozem has been studied under laboratory conditions. Molecular-biological approaches and bioinformatic methods of analysis were used in the study. The objects of the study were the chernozem samples taken in Voronezh oblast (N 51°1′41″, E 40°43′31″). The phylogenetic and functional diversity of the prokaryotic complex of oil-contaminated chernozem was studied, when introducing potassium nitrate and potassium chloride under conditions of a slightly alkaline reaction of the medium. Contamination of chernozem with oil in concentration 5% of the soil mass led to alkalinization of the medium from 7.1 to 7.9 pH values. The introduction of potassium nitrate and chloride, both separately and together in a total dose of 2 mmol/100 g soil removed this negative effect. The combined addition of potassium nitrate and potassium chloride led to a more than twofold increase in the biomass of metabolically active prokaryotic cells and the number of copies of functional genes responsible for the synthesis of alkanmonooxygenase enzymes involved in the decomposition of oil. In the presence of oil, the formation was revealed of a specific complex of bacteria, in which representatives of Actinobacteria (Rhodococcus erythropolis) and Alphaproteobacteria (Bradyrhizobium japonicum) dominated. Rhodococcus erythropolis and Bradyrhizobium japonicum, being autochthonous organisms in uncontaminated soil, began to occupy dominant positions in oil-contaminated samples, and the introduction of nitrates enhanced this effect.
- Subjects
VORONEZH (Russia); POTASSIUM nitrate; POTASSIUM chloride; RHODOCOCCUS erythropolis; NITROGEN fertilizers; SOIL biology
- Publication
Eurasian Soil Science, 2023, Vol 56, Issue 7, p924
- ISSN
1064-2293
- Publication type
Article
- DOI
10.1134/S1064229323600641