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- Title
Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress.
- Authors
Delatte, Benjamin; Jeschke, Jana; Defrance, Matthieu; Bachman, Martin; Creppe, Catherine; Calonne, Emilie; Bizet, Martin; Deplus, Rachel; Marroquí, Laura; Libin, Myriam; Ravichandran, Mirunalini; Mascart, Françoise; Eizirik, Decio L.; Murrell, Adele; Jurkowski, Tomasz P.; Fuks, François
- Abstract
The TET enzymes convert methylcytosine to the newly discovered base hydroxymethylcytosine. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain, and results lack in vivo models. Here we show a global decrease of hydroxymethylcytosine in cells treated with buthionine sulfoximine, and in mice depleted for the major antioxidant enzymes GPx1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in coding genes, and intriguingly in microRNA genes, both involved in response to oxidative stress. These results thus suggest a profound effect of in vivo oxidative stress on the global hydroxymethylome.
- Subjects
METHYLCYTOSINE; OXIDATIVE stress; SULFOXIMINES; MICRORNA; GENETIC code
- Publication
Scientific Reports, 2015, p12714
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep12714