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- Title
The mouse hairy ears mutation exhibits an extended growth (anagen) phase in hair follicles and altered Hoxc gene expression in the ears.
- Authors
Mentzer, Sarah E.; Sundberg, John P.; Awgulewitsch, Alexander; Chao, Hanna H. J.; Carpenter, Donald A.; Wei-Dong Zhang; Rinchik, Eugene M.; Yun You
- Abstract
The mouse In(15)2Rl (hairy ears, Eh) mutation is a paracentric inversion of the distal half of chromosome 15 (Chr 15). Heterozygous Eh/+ mice display misshaped and hairy ears that have more and longer hair than the ears of their wild-type littermates. We mapped, cloned and sequenced both inversion breakpoints. No protein-coding transcript was disrupted by either breakpoint. The proximal breakpoint is located between syntrophin basic 1 ( Sntb1) and hyaluronan synthase 2 ( Has2), and the distal breakpoint maps between homeobox C4 ( Hoxc4) and single-strand selective monofunctional uracil DNA glycosylase ( Smug1), near the middle and the telomere ends of Chr 15, respectively. The inversion spans ~47 megabases. Our genetic analysis suggests that the hairy-ear phenotype is caused by the proximal breakpoint of the inversion-bearing Chr 15. Quantitative RNA analysis by real-time polymerase chain reaction for the genes flanking the breakpoint indicated no changes in expression levels except for some homeobox C ( Hoxc) genes whose expression was elevated in developing and mature skin of the ears but not of other body regions. The increased hair length on the ears of Eh/+ mice was due to an extension of the anagen stage in the hair cycle, as determined by histological analysis. Our data indicate that the Eh phenotype arises from mis-expression of Hoxc genes.
- Subjects
GENETIC mutation; MICE; HAIR; EAR; ANIMAL mutation; HYALURONIC acid
- Publication
Veterinary Dermatology, 2008, Vol 19, Issue 6, p358
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/j.1365-3164.2008.00709.x