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- Title
FC-54 Evaluation of IgE-mediated late-phase reactions in the skin of normal placebo- and prednisolone-treated dogs: cellular, cytokine and chemokine responses.
- Authors
Pucheu-Haston, C.; Shuster, D.; Olivry, T.; Brianceau, P.; Lockwood, P.; McClanahan, T.; de Waal Malefyt, R.; Mattson, J.; Hammerberg, B.
- Abstract
IgE-mediated late-phase reactions can be induced in the skin of normal and atopic dogs by intradermal injections of anti-IgE antibody. The histology of these reactions is very similar to that of naturally occurring atopic dermatitis. To characterize the cellular, cytokine and chemokine responses in the skin of placebo- and prednisolone-treated dogs, normal beagles received either placebo or 0.5 mg/kg prednisolone twice daily for three days prior to intradermal injection of polyclonal rabbit anti-canine IgE. Eight-millimetre punch biopsy skin samples were taken before injection and at the injection sites after 6, 24 and 48 h. Histological and immunohistochemical examination revealed a rapid cellular influx. Eosinophil and neutrophil numbers increased from <1 to 61.4 ± 14.1, and from 7 to 62.2 ± 10.8 cells/mm2, respectively, within 6 h after injection , and remained moderately elevated 48 h later. The numbers of CD1c+, CD3+ and CD4+ mononuclear cells were also increased by 6 h. Taqman analysis demonstrated 2.5- to 72-fold increases in mRNA expression for IL-13, IL-5, MCP (CCL2), RANTES (CCL5) and TARC (CCL17). Levels of mRNA for IL-2, IL-4, IL-6 , and IFNγ remained negligible. Prednisolone administration suppressed the influx of neutrophils and eosinophils, and the expression of IL-13, CCL2, CCL5 and CCL17 (33, 97, 58, 86, 73 and 90%, respectively), as well as the influx of CD1c+ and CD3+ cells. These data document the cytokine and chemokine response to anti-IgE injection and demonstrate the anti-inflammatory effect of prednisolone. Funding: Schering-Plough Animal Health.
- Subjects
IMMUNOGLOBULIN E; ATOPIC dermatitis; DOGS; SKIN inflammation; MESSENGER RNA; LEUCOCYTES; DIAGNOSIS
- Publication
Veterinary Dermatology, 2004, Vol 15, p38
- ISSN
0959-4493
- Publication type
Abstract
- DOI
10.1111/j.1365-3164.2004.411_54.x