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- Title
Identification and molecular tagging of a stripe rust resistance gene in wheat line P81.
- Authors
Pu, Z. J.; Chen, G. Y.; Wei, Y. M.; Yang, W. Y.; Yan, Z. H.; Zheng, Y. L.
- Abstract
With 3 figures and 3 tables Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat ( Triticum aestivum L.). With the objective of identifying and tagging a new gene for resistance to stripe rust in wheat line P81, F1, F2 and F2:3 populations from the cross ‘Chuanmai 28’/P81 were inoculated with Chinese PST race CYR32 in greenhouse and field trials. P81 carried a single dominant gene for resistance (designated YrP81) to CYR32. Tests of allelism showed that YrP81 was different from Yr5, Yr10, Yr15 and Yr26. Simple sequence repeat (SSR) and resistance gene-analogue polymorphism (RGAP) between the parents were used for genotyping the F2 populations. YrP81 was closely linked to four SSR loci on chromosome 2BS with genetic distances of 18.3 cM ( Xwmc25), 1.8 cM ( Xgwm429), 4.1 cM ( Xwmc770) and 5.3 cM ( Xgwm148). Two RGAP markers RGA1 (NLRR/XLRR) and RGA2 (Pto kin4/NLRR-INV2) were also closely linked to YrP81 with genetic distances of 4.7 and 6.3 cM, respectively. The linkage map of YrP81 and molecular markers was established in the order Xwmc25- RGA2- RGA1- Xgwm429- YrP81- Xwmc770- Xgwm148. Pedigree analysis, response patterns with Chinese PST races and associations with markers suggested that YrP81 is a novel stripe rust resistance gene. The PCR-based microsatellite and RGAP markers identified here could be applied in selection of YrP81 in wheat breeding.
- Subjects
WHEAT; STRIPE rust; GRASS diseases &; pests; WHEAT rusts; DISEASE resistance of plants
- Publication
Plant Breeding, 2010, Vol 129, Issue 1, p53
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2009.01649.x