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- Title
Removal of bisphenol A in canned liquid food by enzyme-based nanocomposites.
- Authors
Tapia-Orozco, Natalia; Meléndez-Saavedra, Fanny; Figueroa, Mario; Gimeno, Miquel; García-Arrazola, Roeb
- Abstract
Laccase from Trametes versicolor was immobilized on TiO2 nanoparticles; the nanocomposites obtained were used for the removal of bisphenol A (BPA) in a liquid food matrix. To achieve a high enzymatic stability over a wide pH range and at temperatures above 50 °C, the nanocomposite structures were prepared by both physical adsorption and covalent linking of the enzyme onto the nanometric support. All the nanocomposite structures retained 40% of their enzymatic activity after 60 days of storage. Proof-of-concept experiments in aqueous media using the nanocomposites resulted on a > 60% BPA removal after 48 h and showed that BPA was depleted within 5 days. The nanocomposites were tested in canned liquid food samples; the removal reached 93.3% within 24 h using the physically adsorbed laccase. For the covalently linked enzyme, maximum BPA removal was 91.3%. The formation of BPA dimers and trimers was observed in all the assays. Food samples with sugar and protein contents above 3 and 4 mg mL−1 showed an inhibitory effect on the enzymatic activity.
- Subjects
BISPHENOL A; FLUID foods; ENZYME analysis; NANOCOMPOSITE materials; TRAMETES versicolor; LACCASE
- Publication
Applied Nanoscience, 2018, Vol 8, Issue 3, p427
- ISSN
2190-5509
- Publication type
Article
- DOI
10.1007/s13204-018-0675-2