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- Title
LsrB-based and temperature-dependent identification of bacterial AI-2 receptor.
- Authors
Zhang, Yuxi; Qi, Kezong; Jing, Yawei; Zuo, Jiakun; Hu, Jiangang; Lv, Xiaolong; Chen, Zhaoguo; Mi, Rongsheng; Huang, Yan; Yu, Shengqing; Han, Xiangan
- Abstract
The luxS gene is required for autoinducer-2 (AI-2) synthesis in many bacterial species. AI-2 is taken up by a specific receptor to regulate multiple bacterial activities. However, the lack of methods to identify AI-2 receptors has impeded investigations into the roles of AI-2. Here, a luxS mutant of Escherichia coli strain BL21 (DE3) was constructed (named BL21∆luxS), and the recombinant LsrB protein of Salmonella enterica was expressed in BL21∆luxS and BL21 cells, which were named LsrB (BL21∆luxS) and LsrB (BL21), respectively. The results of the activity of recombinant LsrB binding showed that LsrB (BL21) bound to endogenous AI-2 (produced from BL21 strain), while LsrB (BL21∆luxS) did not (as BL21∆luxS cannot produce AI-2). However, the results of recombinant LsrB binding showed that LsrB (BL21∆luxS) can bind exogenous AI-2, which was released from LsrB (BL21∆luxS) at 55 °C for 10 min, while LsrB (BL21) could not bind exogenous AI-2 (due to binding of endogenous AI-2 before). Furthermore, analysis of the thermal stability of AI-2 showed that that AI-2 activity was relatively high at incubation temperatures below 65 °C. These findings will be beneficial for screening of new AI-2 receptors in different bacterial species.
- Subjects
ESCHERICHIA coli; EFFECT of heat on bacteria; BACTERIAL genetics; RECOMBINANT proteins; GENETIC testing
- Publication
AMB Express, 2017, Vol 7, Issue 1, p1
- ISSN
2191-0855
- Publication type
Article
- DOI
10.1186/s13568-017-0486-y