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- Title
CD70-expressing CD4 T cells produce IFN-γ and IL-17 in rheumatoid arthritis.
- Authors
Park, Jin Kyun; Han, Bobby Kwanghoon; Park, Ji Ah; Woo, Youn Jung; Kim, So Young; Lee, Eun Young; Lee, Eun Bong; Chalan, Paulina; Boots, Annemieke M.; Song, Yeong Wook
- Abstract
Objective. CD70-expressing CD4 T cells are enriched in RA and promote autoimmunity via co-stimulatory CD70-CD27 interaction. This study aimed to explore the phenotype and cytokine production of CD70+ CD4 T cells in RA.Methods. Peripheral blood mononuclear cells from 32 RA patients were isolated and frequencies of CD70+ cells within different CD4 T subsets were analysed using flow cytometry. IFN-γ and IL-17 production were compared between the CD70+ and CD70− cells. Expression of master transcription factors T-bet, GATA3 and retinoic acid–related orphan receptor gamma t (RORγt) were examined by real-time PCR. Results are presented as mean (s.e.m.).Results. CD4 T cells of healthy controls rarely expressed CD70 as compared with CD4 T cells of RA patients [mean 0.9% (s.e.m. 0.3%) vs 7.6 (0.6), P < 0.001]. In RA, CD70+ cells were present within all CD4 T cell subsets, i.e. CD45RA+CCR7+ naive, CD45RA−CCR7+ central memory, CD45RA−CCR7− effector memory and CD45RA+CCR7− terminally differentiated effector memory T cells with a mean frequency of 3.9% (s.e.m. 1.1%), 4.0 (0.5), 4.2 (0.7) and 9.4 (4.3), respectively. As compared to CD70− CD4 T cells, CD70+ CD4 T cells produced significantly more IFN-γ and IL-17 after short activation. CD70+ CD4 T cells preferentially expressed transcription factor RORγt.Conclusion. CD70+ CD4 T cells are enriched in RA and may directly contribute to RA pathogenesis by producing IFN-γ and IL-17. Targeting CD70+ CD4 T cells might offer new therapeutic opportunities in RA.
- Subjects
SOUTH Korea; T cells; ACADEMIC medical centers; FLOW cytometry; INTERFERONS; INTERLEUKINS; POLYMERASE chain reaction; RESEARCH funding; RHEUMATOID arthritis; STATISTICS; T-test (Statistics); DATA analysis; DATA analysis software; PHYSIOLOGY
- Publication
Rheumatology, 2014, Vol 53, Issue 10, p1896
- ISSN
1462-0324
- Publication type
Article
- DOI
10.1093/rheumatology/keu171