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- Title
Molecular and cytogenetic changes involved in the immortalization of nasopharyngeal epithelial cells by telomerase.
- Authors
Li, H.M.; Man, C.; Jin, Y.; Deng, W.; Yip, Y.L.; Feng, H.C.; Cheung, Y.C.; Lo, K.W.; Meltzer, P.S.; Wu, Z.G.; Kwong, Y.L.; Yuen, A.P.W.; Tsao, S.W.
- Abstract
Nasopharyngeal carcinoma (NPC) is a common disease in Hong Kong and southern provinces of China. EBV infection is believed to play a critical role in the development of NPC. Previous studies on the transformation mechanism of EBV genes were mostly performed in either NPC or nonnasopharyngeal epithelial cells which may not be representative of premalignant nasopharyngeal epithelial cells. Establishment of a representative cell system would greatly facilitate the elucidation of the role of EBV infection in the development of NPC. Using telomerase alone, we were able to establish an immortalized nasopharyngeal epithelial cell line from primary nonmalignant nasopharyngeal biopsies. The telomerase-immortalized nasopharyngeal epithelial cells are largely diploid in karyotype. Interestingly, this newly immortalized nasopharyngeal epithelial cell line, referred as NP460hTert, harbors genetic alterations previously identified in premalignant and malignant nasopharyngeal epithelial cells. These include inactivation of p16 by homozygous deletion of the p16
- Publication
International Journal of Cancer, 2006, Vol 119, Issue 7, p1567
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.22032