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- Title
scm<sup>6</sup>A-seq reveals single-cell landscapes of the dynamic m<sup>6</sup>A during oocyte maturation and early embryonic development.
- Authors
Yao, Huan; Gao, Chun-Chun; Zhang, Danru; Xu, Jiawei; Song, Gege; Fan, Xiu; Liang, Dao-Bo; Chen, Yu-Sheng; Li, Qian; Guo, Yanjie; Cai, Yu-Ting; Hu, Lulu; Zhao, Yong-Liang; Sun, Ying-Pu; Yang, Ying; Han, Jianyong; Yang, Yun-Gui
- Abstract
N6-methyladenosine (m6A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis and embryogenesis. However, the landscape and function of m6A at single cell resolution have not been extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell m6A sequencing (scm6A-seq) method to simultaneously profile the m6A methylome and transcriptome in single oocytes/blastomeres of cleavage-stage embryos. We found that m6A deficiency leads to aberrant RNA clearance and consequent low quality of Mettl3Gdf9 conditional knockout (cKO) oocytes. We further revealed that m6A regulates the translation and stability of modified RNAs in metaphase II (MII) oocytes and during oocyte-to-embryo transition, respectively. Moreover, we observed m6A-dependent asymmetries in the epi-transcriptome between the blastomeres of two-cell embryo. scm6A-seq thus allows in-depth investigation into m6A characteristics and functions, and the findings provide invaluable single-cell resolution resources for delineating the underlying mechanism for gametogenesis and early embryonic development. Modification of RNA with N6-methyladenosine can regulate RNA metabolism. Here they developed scm6A-seq to profile the methylome and transcriptome in single cells, and reveal the functions of m6A modification during oocyte maturation and early embryo development.
- Subjects
EMBRYOLOGY; OVUM; RNA metabolism; RNA modification &; restriction; BLASTOMERES; SYMMETRY (Biology)
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-35958-7