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- Title
Comparative secretome analysis of Fusarium sp. Q7-31T during liquid fermentation using oat straw as a carbon source.
- Authors
Tian, Fei; Xie, Zhan-ling; Zhao, Lian-zheng; Guo, Jing; Han, Xing-bao; Xie, Li-fang; Wang, Yan; Chang, Xin-yuan
- Abstract
Q7-31T was screened from seven strains with high plant cell wall (PCW)-degrading enzyme activities and identified as Fusarium sp. based on the morphological characteristics and internal transcribed spacer (ITS) rDNA sequence analysis. The protein composition of the secretome produced by Q7-31T grown in a liquid medium with oat straw powders or glucoses as a carbon source was investigated using two-dimensional electrophoresis coupled with tandem mass spectrometry. A total of 28 protein species were identified among 115 spots that only existed or had a larger quantity (threefold or more) in the induction medium. As expected, most of them were potentially involved in PCW degradation. Six different superfamilies of glucoside hydrolases (GH5, GH7, GH10, GH13, GH18, and PL1) were identified, and proteases, oxidases, reductases, phospholipases, transferases, and proteins with unknown functions were also detected.
- Publication
Annals of Microbiology, 2015, Vol 65, Issue 4, p2131
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-015-1051-z