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- Title
Expression of recombinant human interleukin-32 in Pleurotus eryngii.
- Authors
Chung, Sang; Kim, Seung; Sapkota, Kumar; Choi, Bong-Suk; Shin, Changho; Kim, Sung-Jun
- Abstract
Interleukin-32 is a novel human cytokine implicated in several inflammatory and autoimmune diseases. Recombinant interleukin -32 can be produced and found to be useful in many fields. Until now, there has been no report on recombinant hIL-32 expressed in basidomycete fungus, Pleurotus eryngii. In this study, we examined whether the popular edible mushroom Pleurotus eryngii could support the expression of novel protein hIL-32. A binary vector pCAMBIA1304 containing the hIL-32 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. The expression of hIL-32 was confirmed by PCR, Southern blot and western blot analysis. The recombinant hIL-32 reached a maximum expression level of 1.9 % of total soluble protein in transgenic mycelia. These results suggest that Pleurotus eryngii expression system can be effective for the production of rhIL-32 at an economically relevant level.
- Publication
Annals of Microbiology, 2011, Vol 61, Issue 2, p331
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-010-0146-9