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- Title
Diagnosis of human immunodeficiency virus infection by immunoassay using a molecularly cloned and expressed virus envelope polypeptide. Comparison to Western blot on 2707 consecutive serum samples.
- Authors
Burke, Donald S.; Brandt, Brenda L.; Redfield, Robert R.; Lee, Tun-Hou; Thorn, Richard M.; Beltz, Gerald A.; Hung, Chung-Ho; Burke, D S; Brandt, B L; Redfield, R R; Lee, T H; Thorn, R M; Beltz, G A; Hung, C H
- Abstract
To detect human immunodeficiency virus (HIV) antibodies in a simple enzyme-linked immunoassay (CBre3-EIA), we used an Escherichia coli-expressed polypeptide antigen, representing the carboxy-terminal third of the external membrane glycoprotein gene fused with the amino-terminal half of the transmembrane glycoprotein gene. Over a 3-month period, 2707 consecutive serum samples referred for confirmatory testing for human T-lymphotrophic virus type III (HTLV-III) antibodies were evaluated by both Western blot and CBre3-EIA. On a single determination for each sample, the CBre3-EIA was found to have an estimated sensitivity (99.9%) and specificity (99.1%) similar or superior to the more cumbersome Western blot method. This study shows that all HIV-seropositive subjects have antibodies to the virus envelope protein; no other virus antigens are required for construction of highly sensitive immunoassays.
- Subjects
HIV; ENZYME-linked immunosorbent assay; IMMUNOASSAY; AIDS diagnosis; ANALYTICAL chemistry techniques; COMPARATIVE studies; IMMUNOENZYME technique; IMMUNOLOGY technique; RESEARCH methodology; MEDICAL cooperation; PROTEINS; RECOMBINANT proteins; RESEARCH; VIRAL antibodies; VIRAL antigens; EVALUATION research
- Publication
Annals of Internal Medicine, 1987, Vol 106, Issue 5, p671
- ISSN
0003-4819
- Publication type
journal article
- DOI
10.7326/0003-4819-106-5-671