We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Tissue engineering of nanosilver-embedded peripheral nerve scaffold to repair nerve defects under contamination conditions.
- Authors
Tan Ding; Jun-Bin Yin; Hu-Ping Hao; Chao Zhu; Ting Zhang; Ya-Cheng Lu; Li-Ying Wang; Zhe Wang; Yun-Qing Li; Ding, Tan; Yin, Jun-Bin; Hao, Hu-Ping; Zhu, Chao; Zhang, Ting; Lu, Ya-Cheng; Wang, Li-Ying; Wang, Zhe; Li, Yun-Qing
- Abstract
<bold>Introduction: </bold>We employed a nanosilver-collagen scaffold and tested its effects on inhibiting bacteria and facilitating nerve regeneration.<bold>Methods: </bold>Based on our previous research, we prepared bionic scaffolds with different concentrations of nanosilver and examined their internal structures by scanning electron microscopy and energy dispersive spectroscopy. We implanted these scaffolds or autologous nerve grafts into rats to repair a 10-mm injury of the sciatic nerve.<bold>Results: </bold>The 2 mg/ml group showed a >10 mm bacterial inhibition zone in all 3 types of bacterial culture dishes. At day 60 postsurgery, the 2 mg/ml group also showed the highest amplitude of evoked potential (AMP) and nerve conduction velocity (NCV). The regenerating nerves in the 2 mg/ml group were denser and more mature, and with thicker and well-arrayed myelin sheath.<bold>Conclusions: </bold>These results demonstrate that nanosilver scaffolds (2 mg/ml group) were effective in inhibiting bacteria both in vitro and in vivo, and reduced the contamination-caused immune responses, which in turn promoted nerve regeneration and functional recovery.
- Subjects
SCIATIC nerve surgery; SCIATIC nerve injuries; ANIMAL experimentation; ANTHROPOMETRY; BIOLOGICAL models; CULTURE media (Biology); ELECTRON microscopy; ESCHERICHIA coli; NANOPARTICLES; NERVOUS system regeneration; PSEUDOMONAS; RATS; SCIATIC nerve; SILVER; STAPHYLOCOCCUS aureus; TISSUE engineering; SKELETAL muscle; LEUKOCYTE count
- Publication
International Journal of Artificial Organs, 2015, Vol 38, Issue 9, p508
- ISSN
0391-3988
- Publication type
journal article
- DOI
10.5301/ijao.5000439