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- Title
Pegylated Interferon-α-Induced Natural Killer Cell Activation Is Associated With Human Immunodeficiency Virus-1 DNA Decline in Antiretroviral Therapy-Treated HIV-1/Hepatitis C Virus-Coinfected Patients.
- Authors
Hua, Stéphane; Vigano, Selena; Tse, Samantha; Zhengyu, Ouyang; Harrington, Sean; Negron, Jordi; Garcia-Broncano, Pilar; Marchetti, Giulia; Genebat, Miguel; Leal, Manuel
- Abstract
Background. Interferon alpha (IFN-α) can potently reduce human immunodeficiency virus type 1 (HIV-1) replication in tissue culture and animal models, but may also modulate residual viral reservoirs that persist despite suppressive antiretroviral combination therapy. However, mechanisms leading to viral reservoir reduction during IFN-α treatment are unclear. Methods. We analyzed HIV-1 gag DNA levels in CD4 T cells by digital droplet polymerase chain reaction and CD8 T-cell and natural killer (NK) cell phenotypes by flow cytometry in a cohort of antiretroviral therapy-treated HIV-1/hepatitis C virus- coinfected patients (n = 67) undergoing treatment for hepatitis C infection with pegylated IFN-a and ribavirin for an average of 11 months. Results. We observed that IFN-a treatment induced a significant decrease in CD4 T-cell counts (P < .0001), in CD4 T-cell- associated HIV-1 DNA copies (P = .002) and in HIV-1 DNA copies per microliter of blood (P < .0001) in our study patients. Notably, HIV-1 DNA levels were unrelated to HIV-1-specific CD8 T-cell responses. In contrast, proportions of total NK cells, CD56brightCD16- NK cells, and CD56brightCD16+ NK cells were significantly correlated with reduced levels of CD4 T-cell-associated HIV-1 DNA during IFN-a treatment, especially when coexpressing the activation markers NKG2D and NKp30. Conclusions. These data suggest that the reduction of viral reservoir cells during treatment with IFN-a is primarily attributable to antiviral activities of NK cells.
- Subjects
THERAPEUTIC use of interferons; RIBAVIRIN; BIOMARKERS; CELL receptors; DNA; FLOW cytometry; HEPATITIS C; HIV; HIV infections; KILLER cells; POLYMERASE chain reaction; T cells; PHENOTYPES; ANTIRETROVIRAL agents; TREATMENT duration; MIXED infections; PHARMACODYNAMICS
- Publication
Clinical Infectious Diseases, 2018, Vol 66, Issue 12, p1910
- ISSN
1058-4838
- Publication type
Article
- DOI
10.1093/cid/cix1111