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- Title
Amino acids of coxsackie B5 virus are critical for infection of the murine insulinoma cell line, MIN-6.
- Authors
Al-Hello, Haider; Ylipaasto, Petri; Smura, Teemu; Rieder, Elisabeth; Hovi, Tapani; Roivainen, Merja
- Abstract
It was shown recently that 15 successive passages of a laboratory strain of the Coxsackie B virus 5 in a mouse pancreas (CBV-5-MPP) resulted in apparent changes in the virus phenotype, which led to the capacity to induce a diabetes-like syndrome in mice. For further characterization of islet cell interactions with a passaged virus strain, a murine insulinoma cell line, MIN-6, was selected as an experimental model. The CBV-5-MPP virus strain was not able to replicate in MIN-6 cells in vitro but required adaptation over a few days for progeny production and the generation of cytopathic effects. In order to determine the genetic characteristics required for virus growth in MIN-6 cells, the whole genome of the MIN-6-adapted virus variant was sequenced, and critical amino acids were identified by comparing the sequence with that of a virus strain passaged repeatedly in the mouse pancreas. The results of site-directed mutagenesis demonstrated that only one residue, amino acid 94 of VP1, is a major determinant for virus adaptation to MIN-6 cells. J. Med. Virol. 81:296-304, 2009. © 2008 Wiley-Liss, Inc.
- Publication
Journal of Medical Virology, 2009, Vol 81, Issue 2, p296
- ISSN
0146-6615
- Publication type
Article
- DOI
10.1002/jmv.21391