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- Title
Fluorescence Studies on a Designed Peptide of REIP as a Potential Hydrophobic Drug Carrier.
- Authors
Yongzhi Lu; Xiaojun Zhao
- Abstract
Many hydrophobic drugs have limited or no activity in clinical application due to their poor aqueous solubility. In order to find fine delivery system, we designed a novel peptide REIP (Ac-RIEIRIEIAPAIEIRIEIR) and investigated its properties as a delivery system. Microcrystal of pyrene could be stabilized by the peptide REIP and suspended in aqueous solution. We used pyrene as a model hydrophobic drug and egg phosphatidylcholine (EPC) vesicles as model membranes to study the ability of REIP in hydrophobic drug encapsulation and transfer to EPC vesicles (liposome) by analyzing the fluorescence spectroscopy of pyrene. It was found that pyrene was present in the crystalline form when stabilized by REIP and was able to transfer into EPC vesicles in molecular form. The concentration of pyrene released into the EPC vesicles at a given time was quantified using a calibration curve. Double exponential curve fitting was better for the release profiles of REIP-Py solution than single exponential curve fitting.
- Subjects
PEPTIDES; PYRENE; LECITHIN; FLUORESCENCE spectroscopy; LUMINESCENCE spectroscopy
- Publication
International Journal of Peptide Research & Therapeutics, 2011, Vol 17, Issue 2, p81
- ISSN
1573-3149
- Publication type
Article
- DOI
10.1007/s10989-011-9245-0