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- Title
Higher expression and activity of metalloproteinases in human cervical carcinoma cell lines is associated with HPV presence.
- Authors
Da Silva Cardeal, Laura Beatriz; Brohem, Carla Abdo; Correâa, Tatiana Caroline Silveira; Winnischofer, Sheila Maria Brochado; Nakano, Fabio; Boccardo, Enrique; Villa, Luisa Lina; Sogayar, Mari Cleide; Maria-Engler, Silvya Stuchi
- Abstract
Matrix metalloproteinases (MMPs) MMP–2, MMP–9, and MT1-MMP are required for basement membrane degradation in cervical carcinoma. We evaluated the expression and activity of MMPs and their inhibitors RECK and TIMP-2 in 3 human invasive cervical carcinoma cell lines. Two HPV16-positive cell lines (SiHa and CaSki) and an HPV-negative cell line (C33A) were cultured either onto a type-I collagen gel, Matrigel™, or plastic, to recreate their three-dimensional growth environment and evaluate the expression of these genes using quantitative real-time PCR. We also analyzed the gelatinolytic activity of MMP-2 and MMP–9 by zymography. We found that HPV (human papillomavirus)-positive cell lines express higher levels of MMP-2, MT1-MMP, and TIMP-2 than the HPV negative cell line. In addition, MMP-9 was expressed at very low levels in both HPV-negative and HPV-positive cell lines. We also observed that the expression of the RECK gene is higher in CaSki cells, being associated with higher pro-MMP-2 activity. Furthermore, Matrigel™ substrate influences MMP-2 expression in both SiHa and CaSki cells. On the other hand, we found that type-I collagen gel, but not Matrigel™, can enhance pro-MMP-2 activity in all cell lines. Our results suggest that the presence of HPV is related to increased expression of MMP-2, MT1-MMP, and TIMP-2, and that pro-MMP-2 activity is higher in HPV-positive than in HPV-negative cells.
- Subjects
METALLOPROTEINASES; METALLOENZYMES; GENE expression; PAPILLOMAVIRUSES; CERVICAL cancer; CELL lines
- Publication
Biochemistry & Cell Biology, 2006, Vol 84, Issue 5, p713
- ISSN
0829-8211
- Publication type
Article
- DOI
10.1139/O06-084