We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Multiplex polymerase chain reaction using ethidium monoazide and propidium monoazide for distinguishing viable and dead cells of arcobacters in biofilm.
- Authors
Hrušková, Lucie; Mot'ková, Petra; Vytřasová, Jarmila
- Abstract
This paper concerns the formation of biofilm in bacteria of the genus Arcobacter. A multiplex polymerase chain reaction (PCR) method was introduced and optimized for detecting biofilm while using the intercalating dyes ethidium monoazide (EMA) and propidium monoazide (PMA), first for analysis of strains of the genus Arcobacter from a collection, and then applied to samples of prepared biofilms. The results of the study indicate considerable variability among species of bacteria within the genus Arcobacter. The EMA-PMA PCR method can distinguish viable cells from dead cells and is therefore suitable for determining the viability of cells.
- Subjects
POLYMERASE chain reaction; ETHIDIUM; AZIDES; PROPIDIUM monoazide; MICROBIAL viability counts; ARCOBACTER; BIOFILMS
- Publication
Canadian Journal of Microbiology, 2013, Vol 59, Issue 12, p797
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2013-0635