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- Title
(-)-Epicatechin maintains endurance training adaptation in mice after 14 days of detraining.
- Authors
Hüttemann, Maik; Lee, Icksoo; Malek, Moh H.
- Abstract
The purpose of this study was to determine whether (-)-epicatechin (mainly found in cocoa) could attenuate detraining effects in the hindlimb muscles of mice. Thirty-two male mice were randomized into 4 groups: control, trained, trained with 14 d of detraining and vehicle (DT-14-W), and trained with 14 d of detraining and (-)-epicatechin [DT-14-(-)-Epi]. DT-14-(-)-Epi received (-)-epicatechin (1.0 mg/kg 2x/d), whereas water was given to the DT-14-W group. The latter 3 groups performed 5 wk of endurance training 5x/wk. Hindlimb muscles were harvested, and Western blots, as well as enzyme analyses, were performed. Training significantly increased capillary-to-fiber ratio (≈78.8%), cytochrome-c oxidase (≈35%), and activity (≈144%) compared to controls. These adaptations returned to control levels for the DT-14-W group, whereas the DT-14-(-)-Epi group was able to maintain capillary-to-fiber ratio (≈44%), CcO protein expression (≈45%), and activity (≈108%) above control levels. In addition, the increase in capillarity was related to decreased protein expression of thrombospondin-1, an antiangiogenic regulator. Furthermore, there were no significant differences in endurance capacity between the trained and DT-14-(-)-Epi groups. Our data suggest that (-)-epicatechin may be a suitable compound to maintain exercise-induced improved capillarity and mitochondrial capacity, even when exercise regimens are discontinued.
- Subjects
COCOA; HINDLIMB; MICE; PROTEINS; WESTERN immunoblotting; IMMUNOBLOTTING
- Publication
FASEB Journal, 2012, Vol 26, Issue 4, p1413
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.11-196154