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- Title
Role of kinesin light chain-2 of kinesin-1 in the traffic of Na,K-ATPase-containing vesicles in alveolar epithelial cells.
- Authors
Trejo, Humberto E.; Lecuona, Emilia; Grillo, Doris; Szleifer, Igal; Nekrasova, Oksana E.; Gelfand, Vladimir I.; Sznajder, Jacob I.
- Abstract
Recruitment of the Na,K-ATPase to the plasma membrane of alveolar epithelial cells results in increased active Na+ transport and fluid clearance in a process that requires an intact microtubule network. However, the microtubule motors involved in this process have not been identified. In the present report, we studied the role of kinesin-1, a plus-end microtubule molecular motor that has been implicated in the movement of organelles in the Na,K-ATPase traffic. We determined by confocal microscopy and biochemical assays that kinesin-1 and the Na,K-ATPase are present in the same membranous cellular compartment. Knockdown of kinesin-1 heavy chain (KHC) or the light chain-2 (KLC2), but not of die light chain-1 (KLC1), decreased die movement of Na,K-ATPase-containing vesicles when compared to sham siRNA-transfected cells (control group). Thus, a specific isoform of kinesin-1 is required for microtubule-dependent recruitment of Na,K-ATPase to the plasma membrane, which is of physiological significance.
- Subjects
SODIUM ions; POTASSIUM; ADENOSINE triphosphatase; CELL membranes; EPITHELIAL cells; KINESIN
- Publication
FASEB Journal, 2010, Vol 24, Issue 2, p374
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.09-137802