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- Title
Spectrofluorimetric study of the interaction of methyl-parathion with fish serum albumin.
- Authors
Silva, Dilson; Cortez-Moreira, Madelayne; Cunha Bastos, Vera Lúcia Freire; Cunha Bastos, Jayme; Martins Cortez, Célia
- Abstract
The interaction of methyl-parathion with the albumin of Piaractus mesopotamicus (Holmberg 1887) (= pacu), a fish species typical of Brazilian rivers, was studied and the results compared with known values for human and bovine albumin obtained in an earlier investigation. Methyl-parathion ( O, O-dimethyl O- p-nitrophenyl phosphorothioate) is an organophosphorous pesticide still used in agriculture and fish farming in many countries. The fluorescence quenching technique with tryptophan as a natural probe was used to detect for the presence of methyl-parathion. Fluorescence can be mathematically expressed by the Stern–Volmer equation to calculate quenching constants, and changes in the behavior of Stern–Volmer curves at different temperatures indicate the nature of the mechanism causing the quenching. Our results indicate that methyl-parathion forms a complex with fish albumin. The estimated association constant is 9.73 × 103 (± 4.9 × 102) M at 25°C.
- Subjects
PARATHION; EFFECT of chemicals on fishes; ALBUMINS in animal nutrition; PHYSIOLOGICAL effects of pesticides; FLUORESCENCE; TRYPTOPHAN
- Publication
Fish Physiology & Biochemistry, 2010, Vol 36, Issue 3, p427
- ISSN
0920-1742
- Publication type
Article
- DOI
10.1007/s10695-009-9312-z