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- Title
Ultrahigh-field DCE-MRI of angiogenesis in a novel angiogenesis mouse model.
- Authors
Wittenborn, Thomas; Nielsen, Thomas; Nygaard, Jens V.; Larsen, Esben K.U.; Thim, Troels; Rydtoft, Louise M.; Vorup-Jensen, Thomas; Kjems, Jørgen; Nielsen, Niels Chr.; Horsman, Michael R.; Falk, Erling
- Abstract
Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents. J. Magn. Reson. Imaging 2012;35:703-710. © 2011 Wiley Periodicals, Inc.
- Publication
Journal of Magnetic Resonance Imaging, 2012, Vol 35, Issue 3, p703
- ISSN
1053-1807
- Publication type
Article
- DOI
10.1002/jmri.22855